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5 edition of Influence of cytokines on multidrug resistance transporters in human hepatoma cell lines found in the catalog.

Influence of cytokines on multidrug resistance transporters in human hepatoma cell lines

Gigi Lee

Influence of cytokines on multidrug resistance transporters in human hepatoma cell lines

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Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.) -- University of Toronto, 2001.

SeriesCanadian theses = -- Theses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches : negative.
ID Numbers
Open LibraryOL20835759M
ISBN 100612631850
OCLC/WorldCa52623201

@article{osti_, title = {Binding diversity of antibodies against external and internal epitopes of the multidrug resistance gene product P-glycoprotein}, author = {Lehne, G and De Angelis, P and Clausen, O P.F. and Egeland, T and Rugstad, H E}, abstractNote = {P-glycoprotein (Pgp) is a trans-membraneous protein that is associated with multidrug resistance (MDR) in human .   Free Online Library: Alopecurone B reverses doxorubicin-resistant human osteosarcoma cell line by inhibiting P-glycoprotein and NF-kappa B signaling.(nuclear factor kappa B, Report) by "Phytomedicine: International Journal of Phytotherapy & Phytopharmacology"; Health, general Biological sciences Science and technology, general .


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Influence of cytokines on multidrug resistance transporters in human hepatoma cell lines by Gigi Lee Download PDF EPUB FB2

Cytokines alter the expression and activity of the multidrug resistance transporters in human hepatoma cell lines; analysis using RT‐PCR and cDNA microarrays Gigi Lee Faculty of Pharmacy, University of Toronto, 19 Russell Street, Toronto, Ontario, Canada M5S 2S2Cited by: Cytokines Alter the Expression and Activity of the Multidrug Resistance Transporters in Human Hepatoma Cell Lines; Analysis Using RT‐PCR and cDNA Microarrays.

Author links open overlay panel Gigi Lee Micheline Piquette‐Miller Cited by: Multidrug-resistant Huh-7 cell lines, developed with increasing concentrations of doxorubicin, cisplatin, carboplatin, mitomycin C, and vincristine, demonstrated a significant differential profile of miRNAs when compared with the parental cell line.

miRb, miRa, miRb-5p, miRd, and miRa were the most differentially expressed, and they are thought to play Author: Baojun Duan, Chen Huang, Jun Bai, Yu Lian Zhang, Xi Wang, Juan Yang, Jun Li.

Pro‐inflammatory cytokines suppress the hepatic expression of the multidrug resistance transporters in rodents, indicating potential usefulness in chemotherapy. Our objective was Influence of cytokines on multidrug resistance transporters in human hepatoma cell lines book investigate their impact in human hepatoma cells.

HuH 7 and HepG2 cells were treated with IL‐1β, IL‐6, or TNF‐α for 0–72 by: Cytokines Alter the Expression and Activity of the Multidrug Resistance Transporters in Human Hepatoma Cell Lines; Analysis Using RT-PCR and cDNA Microarrays.

Pro-inflammatory cytokines suppress the hepatic expression of the multidrug resistance transporters in rodents, indicating potential usefulness in chemotherapy.

The aims of this study were therefore to investigate the effects of cytokines on the expression of transporters, CYPs and chemokine receptors, over a period of 48 h.

This was investigated at both the mRNA and protein level using real-time quantitative polymerase chain reaction (QPCR) and flow cytometry by: Overall survival of patients with hepatocellular carcinoma (HCC) remains poor, and the multidrug resistance of HCC cells contributes to the limited efficacy of anti-cancer drugs.

Tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 are proinflammatory cytokines known to alter expression of drug transporters in rodent liver. However, their effects toward human hepatic transporters remain poorly characterized. in Multidrug Resistance of Neoplastic Cells The human multidrug resistance protein MRP1 is a kDa membrane glycopro­ ies using plasma membrane vesicles prepared from MRPl-overproducing cell lines.

Kvačkajová-Kišucká et al. demonstrated increased ATP-dependent, high-affinity transport activities for cys. Two transmembrane xenobiotic transporter proteins, P glycoprotein (Pgp) and the multidrug resistance protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells in culture (1–5).

Despite these findings, the role these transporters play in clinical drug resistance exhibited by human breast cancer is unclear; hence, alternate or additional drug resistance. Title:ABC Transporters: Regulation and Association with Multidrug Resistance in Hepatocellular Carcinoma and Colorectal Carcinoma VOLUME: 26 ISSUE: 7 Author(s):María Paula Ceballos, Juan Pablo Rigalli, Lucila Inés Ceré, Mariana Semeniuk, Viviana Alicia Catania and María Laura Ruiz* Affiliation:Institute of Experimental Physiology, Influence of cytokines on multidrug resistance transporters in human hepatoma cell lines book of Biochemical and Cited by: 8.

Abstract. The development of multidrug resistance (MDR) to chemotherapy remains a major challenge in the treatment of cancer. Resistance exists against Influence of cytokines on multidrug resistance transporters in human hepatoma cell lines book effective anticancer drug and can develop by numerous mechanisms including decreased drug uptake, increased drug efflux, activation of detoxifying systems, activation of DNA repair mechanisms, Cited by: Multidrug resistance (MDR) is a phenomenon whereby cells confer drug resistance to structurally and functionally unrelated compounds.

P-glycoprotein (P-gp; MDR1 or ABCB1), a member of the ATP-binding cassette transporter superfamily, has been particularly recognized for its contribution to MDR in the field of cancer ed expression of P-gp has Author: Adrian P. Turner, Camille Alam, Reina Bendayan. Cinnamon is one of the oldest herbal medicine that has been historically known to possess has anticancer property.

In this study, the cytotoxicity of the cinnamon extracts towards human breast cancer cell line (MCF-7) was investigated.

Cinnamomum zeylanicum was extracted using Soxhlet and water extraction methods. Normal human epidermal keratinocytes (NHEK) and dermal fibroblasts express a cell-specific pattern of efflux transport proteins.

Since regulatory mechanisms for these transporters in cells of the human skin were unknown, we analyzed the influence of inflammatory cytokines on the expression of multidrug resistance-associated proteins (MRP1, 3, 4, 5).Cited by: Multidrug resistance cell lines selected with either vinblastine, colchicine, or taxol from the drug-sensitive murine macrophage-like cell line J overexpress the.

Normal human epidermal keratinocytes (NHEK) and dermal fibroblasts express a cell-specific pattern of efflux transport proteins. Since regulatory mechanisms for these transporters in cells of the human skin were unknown, we analyzed the influence of inflammatory cytokines on the expression of multidrug resistance-associated proteins.

Multidrug Efflux Pumps. Drug efflux pumps expressed on human cancer cells majorly contribute to MDR (Sharom, ).These efflux pumps belong to ATP-binding cassette (ABC) family and include (a) P-gp also known as multidrug resistance protein 1 (MDR1) or cluster of differentiation (CD) a ATP-binding cassette sub-family B member 1 encoded in human by ABCB1 gene (b) Multidrug Resistance Cited by: However, reduction of multidrug resistance by TNF and other proinflammatory cytokines in hepatoma cells can also reduce the resistance to chemotherapy.

These findings suggest that regulatory changes of the mdr1 gene by TNF appear to be tissue-and cell-specific. The rat mdr1b promoter contains two major regulatory sites: NFκB and pCited by: Multidrug-resistance (MDR) is the chief limitation to the success of chemotherapy.

According to the National Cancer Institute, multidrug-resistance is a phenomenon where cancer cells adopt to anti-tumor drugs in such a way that makes the drugs less effective. Studies have shown that 40% of all human cancers develop MDR. Deaths due to cancer occur in Continue reading "Multidrug-Resistance.

Cui, Y. et al. Drug resistance and ATP-dependent conjugate transport mediated by the apical multidrug resistance protein, MRP2, permanently expressed in human and canine cells. Mol. Pharmacol. 55 Cited by:   The past decade has seen tremendous efforts in the research and development of new chemotherapeutic drugs using target-based approaches.

These efforts have led to the discovery of small molecule tyrosine kinase inhibitors (TKIs). Following the initial approval of imatinib by the US FDA inmore than 15 TKIs targeting different tyrosine kinases have.

Multidrug resistance (MDR) resulting from different defensive mechanisms in cancer is one of the major obstacles of clinical treatment. To circumvent MDR many reversal agents have been developed, but most of them fail in clinical trials due to severely adverse effects.

Recently, certain natural products have been reported to overcome MDR, including Cited by: 6. Cell lines and cell culture.

Human HCC cell line, HepG 2 (GDC), was purchased from the China Center for Type Culture Collection (Wuhan University, Hubei). HepG 2 was induced to form a multidrug resistant cell line (HepG 2 /ADM) by exposure to gradually increased concentration of ADM.

Firstly, eukaryotic expression vector pBK-TNF-α was Cited by: 9. Clinical efficacy of anticancer chemotherapies is dramatically hampered by multidrug resistance (MDR) dependent on inherited traits, acquired defence against toxins, and adaptive mechanisms mounting in tumours. There is overwhelming evidence that molecular events leading to MDR are regulated by redox mechanisms.

For example, chemotherapeutics which overrun the first Cited by: Adv Drug Deliv 3 – [20] Fojo T and Bates S (). Strategies for reversing drug resistance. Oncog – [21] Lee G and Piquette-Miller M ().

Cytokines alter the expression and activity of the multidrug resistance transporters in human hepatoma cell lines; analysis using RT-PCR and cDNA by: The study of multidrug resistance (MDR) in tumor cell lines has led to the discovery of the plasma membrane P-glycoprotein (Pgp) molecule.

This protein functions as an energy-dependent pump for the efflux of diverse anticancer drugs from MDR by: The influence of IU/mL of cytokines (IFNγ, TNFa or IL-2), on the transducted human colon carcinoma cell lines HCT15 and HCT, was investigated for MDR1 gene and P-gp expression (46).

In both cell lines, MDR1 mRNA levels were decreased by the three cytokines in a time-dependent manner, the most striking effect being observed with TNF a. Chan JY, Chu AC, Fung KP. Inhibition of P-glycoprotein expression and reversal of drug resistance of human hepatoma HepG2 cells by multidrug resistance gene (mdr1) antisense RNA.

Life Sci. ;67(17)– PubMed CrossRef Google ScholarCited by: 1. In cisplatin-resistant cell lines, NAGA was significantly downregulated and hypermethylated at its promoter.

Restoration and overexpression of NAGA in cisplatin-resistant lines induced cytotoxicity in response to cisplatin, whereas depletion of NAGA increased cisplatin resistance.

In their study, Cui et al. human hepatoma cell lines (Bohan et al., ; Lee and Piquette-Miller, ). The present study was therefore designed to analyze the effects of TNF-α and IL-6 treatment on expression of major human hepatic transporters, using primary human hepatocytes which are well recognized as a convenient cellular model to study regulatoryCited by: The role of cytokines in hepatocellular carcinoma.

Anuradha Budhu. Liver Carcinogenesis Section, Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA.

Search for more papers by this author. Xin Wei Wang. Corresponding by:   Hepatocellular carcinoma (HCC) is highly chemoresistant and therefore challenges both physicians and patients.

Augmenter of liver regeneration (ALR), previously also known as ‘hepatic stimulator Cited by: 4. The expression of unusual glycan structures is a hallmark of cancer progression, and their functional roles in cancer biology have been extensively investigated in epithelial-to-mesenchymal transition (EMT) models.

EMT is a physiological process involved in embryonic development and wound healing. It is characterized by loss of epithelial cell polarity and cell adhesion, permitting cell Cited by:   Abstract Lipopolysaccharide (LPS) induces hepatocellular downregulation and endocytic retrieval of multidrug resistance protein 2 (Mrp2, Abcc2).

Basolateral Mrp isoforms may compensate for the intracellular metabolic changes in cholestasis. Therefore, the effect of LPS on the zonal localization of Mrp2 and Mrp3 and the expression of Mrp3, Mrp4, Mrp5, and Mrp6.

Introduction. Cancer is the second leading cause of death in the US [].Inabout million people were diagnosed with cancer and million people died from the disease [].Drug resistance and the resulting ineffectiveness of the drug treatment are responsible for up to 90% of the cancer related deaths [].

Drug resistance in cancer is a well-known phenomenon that. The recognition of intra-tumoral cellular heterogeneity has given way to the concept of the cancer stem cell (CSC). According to this concept, CSCs are able to self-renew and differentiate into all of the cancer cell lineages present within the tumor, placing the CSC at the top of a hierarchical tree.

The observation that these cells—in contrast to bulk tumor cells—are able to Author: Lucas-Alexander Schulte, Juan Carlos López-Gil, Juan Carlos López-Gil, Bruno Sainz, Bruno Sainz, Pat.

2. Mechanisms of MDR. The mechanisms of MDR are multifaceted and complex, and include several factors that are summarized in Fig. these factors are: An adenosine 5′-triphosphate (ATP) dependent decrease in cellular drug accumulation in tumor cell lines associated with elevated levels of the kDa drug transporter P-gp, the kDa multidrug resistance Cited by: 5.

Journal Article: Validation of in vitro cell models used in drug metabolism and transport studies; genotyping of cytochrome P, phase II enzymes and drug transporter polymorphisms in the human hepatoma (HepG2), ovarian carcinoma (IGROV-1) and colon carcinoma (CaCo-2, LS) cell lines.

Cell response to a certain cytokine depends on the cell expressing the correct receptor. Even though cytokine receptors activate similar intercellular signal transduction pathways, cell response to a cytokine signal depends on the cell’s transcription factors, chromatin structures and other proteins responsible for the cell’s development.

Diestra JE, Condom E, Del Muro XG, et al. Expression pdf multidrug resistance proteins P-glycoprotein, multidrug resistance protein 1, breast cancer resistance protein and lung resistance related protein in locally advanced bladder cancer treated with neoadjuvant chemotherapy: biological and clinical implications.

J Urol ; –Cited by: 1.The Multidrug Resistance 1 (MDR1; download pdf ABCB1) gene product P-glycoprotein (P-gp), an ATP binding cassette transporter, extrudes multiple endogenous and exogenous substrates from the cell, playing an important role in normal physiology and xenobiotic distribution and bioavailability.

To date, the predominant animal models used to investigate the role of P-gp .P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug ebook protein 1 (MDR1) or ATP-binding ebook sub-family B member 1 (ABCB1) or cluster of differentiation (CD) is an important protein of the cell membrane that pumps many foreign substances out of cells.

More formally, it is an ATP-dependent efflux pump with broad Aliases: ABCB1, ABC20, CD, CLCS.